Histopathology Work Order

In order for your study to be successful, proper fixation methods are required. Please refer to our Histology Guidelines or contact us for help.

Diabetes Research Center (DRC) Investigators

If you are an approved DRC affiliate, please make sure to check the box on submission form for grant tracking purposes.

Histopathology Work Order Form

"*" indicates required fields

Step 1 of 10

10%

Services Request

Please coordinate sample drop off within 24 hours of completing work order

I need...*

Tissue de-calcification

Wet tissue embedded in paraffin

Section and mount paraffin-embedded tissue onto slides

Section and mount frozen tissue onto slides (Cryosectioning)

H&E or a special stain

Immunohistochemistry (IHC) staining

Slides scanned into a digital format

Pathology Consultation

Image analysis with Visiopharm

User access to microscope

Unavailable Service

Rush Order

I need this rushed!

We will prioritize your order and place it ahead of all other work orders in the queue. This is not a guarantee that we will complete the order within a specific timeframe. Please note that RUSH orders will double the final cost of service.

Optional Addons

HIC provided 32G flashdrive ($10)

Billing/Contact Information

Principal Investigator (PI) First Name*

Principal Investigator (PI) Last Name*

PI Email*

Experiment Name*

Please name this work order in a manner that makes sense for you. We will send out work order numbers and this ID in our notifications.

Submitter First Name*

Submitter Last Name*

This will also be the primary contact person.

Submitter Email*

Phone

Client Type*

Non-UW

Are you a Diabetes Research Center (DRC) Affiliate?*

Yes

Only check this box if you are a confirmed affiliate with the Diabetes Research Center (DRC) for grant tracking purposes.

Payment Method*

Purchase Order (PO)

Credit Card

PO #*

PO document*

Accepted file types: pdf, doc, docx, xls, xlsx, pdf, jpg, gif, png, Max. file size: 512 MB.

please upload a copy of your approved PO document here.

Billing Contact Email*

Grant Worktag Number*

Contact your department admin if you are unsure.

Cost Center Worktag Number*

Contact your department admin if you are unsure.

Resource Worktag Number

Contact your department admin if you are unsure.

Activity Worktag Number

Contact your department admin if you are unsure.

PI Approval*

By checking this box, the PI identified herein attests that he/she/they has approved and agrees to pay in full for the services requested on this work order and any other services requested by email through the Histology and Imaging Core.

Sample Information

Species*

Tissue/Organ*

If you need your organ(s) of interest harvested and trimmed, please provide specific instructions in the "Special Instructions" field in the "Embedding" section of the form. This service may incur tech time fees.

Sample ID Manifest*

Accepted file types: xlsx, xls, doc, docx, Max. file size: 512 MB.

Upload a file containing your sample IDs. An excel file is preferred.

Other Files

Drop files here or

Accepted file types: pdf, doc, docx, xls, xlsx, pdf, jpg, gif, png, Max. file size: 512 MB, Max. files: 5.

Please name your documents in a way that communicates their contents.

Embedding

Tissue Fixed with*

Tissue submerged in*

Embedding Orientation*

Special Instructions for Embedding

Sectioning

How was Frozen tissue processed?*

Embedded in OCT at time of freezing

Frozen without OCT

Section Thickness*

3-5µm, 4µm standard.

# of Slides per block*

# Sections per slide*

Max 5 for small tissues, max 2 for larger tissues. The Histotech may reduce the number of sections per slide at their discretion.

Special Instructions for Sectioning

Staining

H&E and Special Stains*

Stain

# slides per sample ID

Add rows for more stain targets by clicking the plus symbol to the right of the row --->

Special Instructions for H&E and Special Stains

IHC Stains*

Stain Target

# slides per sample ID

Labeling type (DAB or Fluorescent)

By default, we utilize an HRP-catalyzed DAB system for visualization under brightfield. Upon request, we can use other color detection systems or multiplex brightfield staining. Email uwhicadmin@uw.edu before submitting a work order to request chromogens besides DAB and multiplex staining. Please specify if you need fluorescent labeling and your preferred fluorophore (if left blank we will contact you for more information).

*Note: we cannot scan fluorescently labeled slides. We have a scope (90i) equipped with a camera and Nikon elements software that you may schedule time on to manually capture images if desired.

Control Sample*

Please specify here which sample ID should be used as the isotype control so we may cut an extra slide. Please consult with us if you are unsure what makes an appropriate isotype control. Typically, the same species, tissue, and gene treatment should suffice. An isotype control is incubated with an antibody of irrelevant target(s) that shares the same heavy and light chain as the target antibody. Isotype controls ensure the observed staining is due to specific antibody binding to the target rather than an artifact.

IHC Stain Special Instructions

Slide Scanning

Scanning Special Instructions

We utilize a Hamamatsu Nanozoomer automated slide scanning instrument with an auto-focusing feature. Uploading a diagram can assist us to select manual focal points if needed. This instrument does not scan fluorescently labeled slides.

Structure identification diagram

Accepted file types: png, jpg, gif, pdf, tiff, Max. file size: 512 MB.

Image Analysis with Visiopharm

Instructions and/or needs

Visiopharm is equipped with standard binary and region-of-interest tools for quantification as well as machine learning and AI functionalities for more involved studies. Our typical analysis consist of generating a ratio of positive stain to total tissue area. Please describe the measurements you're interested in so the image analyst can make suggestions and develop an appropriate APP for your study. Advanced identification and quantification methodologies will require a consult with the image analyst.

Luminex

Investigator MUST email us prior to bringing samples.

Vendor

Number of Analytes

In-Situ Hybridization (ISH)

ISH has multiple prerequisites to be successful. Email uwhicadmin@uw.edu for consultation before fixing your tissue, if possible.
The investigator must purchase the probe from ACDBio and verify with the manufacturer that it is compatible with the Leica Bond Rx. We supply the positive, negative, and ISH kits for use on our Leica Bond Rx. Email us if you need assistance acquiring a compatible probe.

Probe Target

Number of Slides