Department of Comparative Medicine

Histology and Imaging Core (HIC)

Optimized Antibodies

The HIC utilizes automated immunohistochemistry stainers which provide efficiency and consistency as well as standardization of protocols across studies of all sizes.  Please review the list of over 100 antibodies that have been optimized in our core.  

Please feel free to consult with HIC staff to discuss antibody selection, fixation techniques and control tissue for any IHC project.  Some antibodies are simply not effective for immunohistochemistry techniques and the HIC staff can help you determine the best marker for your study needs.   We are constantly optimizing new (to our core) antibodies so please contact Brian Johnson if an antibody you are interested in is not on our optimized list.

 

AntibodyCatalog NumberCompanyRaised in:Ractive to:Datasheet link
AF-488A-11001Life TechnologiesGoatMshttp://products.invitrogen.com/ivgn/product/A11001
AF-546A-11010Life TechnologiesGoatRbhttp://products.invitrogen.com/ivgn/product/A11010?ICID=search-a11010
AF-594A11062InvitrogenRabbitMshttp://products.invitrogen.com/ivgn/product/A11062?ICID=search-a11062
Annexin A260051-1-IgProteinTechMouseHu, Ms 
ANPab91250AbcamRabbitHu, Ms, Mkhttp://www.abcam.com/anp-antibody-n-terminal-ab91250.html
APOMLS-B5901Lifespan BioscienceGoatMs, Huhttp://www.lsbio.com/Documents/PDF/Antibodies/141308
Arginase 1sc-20150Santa Cruz BiotechnologyRabbitHu, Ms, Rthttp://datasheets.scbt.com/sc-20150.pdf
Armenian Hamster 2°127-035-160Jackson ImmnunoResearchGoatA. Hamsterhttps://www.jacksonimmuno.com/catalog/quickfind
B-Cateninab16051AbcamRabbitMs, Rt, Hu 
BrdUab6326AbcamRatMshttp://www.abcam.com/BrdU-antibody-BU1-75-ICR1-ab6326.html
C-FosPC38CalbiochemRabbitHu,Ms,Rthttp://www.ebiotrade.com/buyf/productsf/calbiochem/pc38.pdf
Calbindin300SwantMouseRbhttp://www.labome.com/product/SWant/300.html
CalbindinCB38SwantRabbitMu, Ms, Rt, Mkhttp://1degreebio.org/reagents/product/504930/?qid=0
CAMPLS-C94098Lifespan BioscienceRabbitMshttp://www.lsbio.com/Documents/PDF/Antibodies/95319
CC3CP229BBioCare MedicalRabbitHu, Mshttp://biocare.net/wp-content/uploads/229.pdf
CCR6MAB195R&D SystemsMouseHuhttp://www.rndsystems.com/Products/MAB195
CD3MCA1477AbD SerotecRatHu, Mshttp://www.abdserotec.com/catalog/datasheet-MCA1477.html
CD3NCL-L-CD3-565LeicaMouseHuhttp://www.leicabiosystems.com/ihc-ish/novocastra-reagents/primary-antibodies/details/product/cd3-1/
CD4550278BD PharmingenRatMshttp://www.bdbiosciences.com/ptProduct.jsp?prodId=18261&key=550278&param=search&mterms=true&from=dTable
CD4VP-C319VectorMouseHuhttp://www.vectorlabs.com/data/protocols/VPC319.pdf
CD414-9766-80eBioscienceRatMshttp://us.ebioscience.com/mouse-cd4-antibody-purified-4sm95.htm
CD8ab4055AbcamRabbitHuhttp://www.abcam.com/cd8-antibody-ab4055.html
CD8a550281BD PharmingenRatMshttp://www.bdbiosciences.com/ptProduct.jsp?prodId=18283&key=550281&param=search&mterms=true&from=dTable
CD8A14-0808-80eBioscienceRatMshttp://us.ebioscience.com/mouse-cd8a-antibody-purified-4sm15.htm
CD8NCL-L-CD8-295LeicaMsHuhttp://www.leicabiosystems.com/ihc-ish-fish/immunohistochemistry-ihc-antibodies-novocastra-reagents/primary-antibodies/details/product/cd8/
CD11cbs-2508RBioss AntibodiesRabbitHuhttp://biossusa.com/store/bs-2508r.html
CD14NCL-L-CD14-223LeicaMouseHuhttp://www.leicabiosystems.com/ihc-ish/novocastra-reagents/primary-antibodies/details/product/cd14/
CD20NCL-L-CD20-L26LeicaMouseHuhttp://www.leicabiosystems.com/ihc-ish/novocastra-reagents/primary-antibodies/details/product/cd20/
CD31ab56299AbcamRatMshttp://www.abcam.com/CD31-antibody-RM0032-1D12-ab56299.htm
CD31PA0250LeicaMouseHuhttp://www.leica-microsystems.com/fileadmin/img_uploads/novocastra_reagents/Novocastra_datasheets/pa0250.pdf
CD31DIA-310DianovaRatMs http://www.dianova.com/en/products/product-highlights/murine-cd31-clone-sz31-formalin-fixed-paraffin-embedded-tissues/
CD45R557390BD PharmingenRatMshttp://www.bdbiosciences.com/ptProduct.jsp?prodId=881&key=557390&param=search&mterms=true
CD45RM0701DakoMouseHuhttp://www.dako.com/us/ar38/p103020/prod_products.htm
CD66b555723BD PharmingenMouseHuhttp://www.bdbiosciences.com/external_files/Doc_Recon_2.0/pm/tds/555723.pdf
CD68MCA1957AbD SerotecRatMshttp://www.abdserotec.com/CodePages/Datasheet.aspx?ProductCode=MCA1957
CD68PA0273LeicaMouseHuhttp://www.leica-microsystems.com/products/total-histology/novocastra-reagents/primary-antibodies/details/product/cd68/
CD138 (Syndecan-1)553712BD PharmingenRatMshttp://www.bdbiosciences.com/ptProduct.jsp?prodId=1716&key=553712&param=search&mterms=true&from=dTable
CD163NCL-L-CD163LeicaMouseHuhttp://www.leicabiosystems.com/fileadmin/img_uploads/novocastra_reagents/Novocastra_datasheets/cd163-l-u.pdf
Claudin-3PA5-16867ThermoScientificRabbitHuhttp://www.pierce-antibodies.com/Claudin-3-antibody-Polyclonal–PA516867.html
Cleaved Notch 14147Cell SignalingRabbitHu, Ms, Rthttp://www.cellsignal.com/products/primary-antibodies/cleaved-notch1-val1744-d3b8-rabbit-mab/4147
CPA1HPA008689SigmaRabbitHuhttp://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=HPA008689|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC
Cytokeratin WSSZ0622DakoRabbitCowhttp://www.dako.com/us/ar38/p104910/prod_products.htm
DesminM0760DakoMouseHuhttp://www.dako.com/us/ar38/p104050/prod_products.htm
E-Cadherin3195Cell SignalingRabbitMs, Huhttp://www.cellsignal.com/products/primary-antibodies/e-cadherin-24e10-rabbit-mab/3195?hit=productId&Ntt=3195
E. coliB0357DakoRabbitHuhttp://www.dako.com/us/ar38/p235383/prod_products.htm
EGFr28-005InvitrogenMouseHuhttp://products.invitrogen.com/ivgn/product/280005?ICID=search-280005
ENT1LSB3385/22621Lifespan BioscienceRabbitHuhttp://www.lsbio.com/Products/Antibodies/AntibodyDetail.aspx?AntibodyID=106033
ESAT6sc-57730Santa Cruz BiotechnologyMouseZFhttp://datasheets.scbt.com/sc-57730.pdf
Estrogen Receptor (ERa)A00010ScyTekMouseHu 
F4/80MF48000InvitrogenRatMshttp://www.invitrogen.com/site/us/en/home.html
FGF9ab71395AbcamRabbitMs, Huhttp://www.abcam.com/fgf9-antibody-ab71395.html
FLAG2368Cell SignalingRabbitMshttp://www.cellsignal.com/products/primary-antibodies/2368
FluB65141GMeridian Life ScienceGoatMshttp://meridianlifescience.com/products/results_2.aspx?searchbox=b65141g&page=1&group=0
Foxp314-4777-82eBioscienceMouseHu 
Foxp3ab20034AbcamMouseHuhttp://www.abcam.com/foxp3-antibody-236ae7-ab20034.html
Foxp314-5773eBioscienceRatMs, Rthttp://us.ebioscience.com/mouse-rat-foxp3-antibody-purified-fjk-16s.htm
Galectin3PA0238LeicaMouseHuhttp://www.leica-microsystems.com/products/total-histology/novocastra-reagents/primary-antibodies/details/product/galectin-3-1/
GFAPZ0334DakoRabbitMswww.dako.com/ar38/p235385/prod_products.htm
GlucagonG2654SigmaMouseRt, Hu 
GlucagonG2654SigmaMouseMs 
Goat Isotype02-6202InvitrogenGoat http://products.invitrogen.com/ivgn/product/026202?CID=Search-02-6202
Goat Secondary305-005-045Jackson ImmnunoResearchRabbitGoathttp://www.jacksonimmuno.com/MERCHANT2/merchant.mv?Screen=PROD&Product_Code=305-005-045
Goat SecondaryG4018SigmaRabbitGoathttp://www.sigmaaldrich.com/catalog/product/sigma/g4018?lang=en&region=US
GR1MAB1037R&D SystemsRatMshttps://www.rndsystems.com/products/mouse-gr-1-ly-6g-antibody-rb6-8c5_mab1037
GranzymeBab4059AbcamRabbitHu, Ms, Rthttp://www.abcam.com/granzyme-b-antibody-ab4059.html
Guinea Pig Secondaryab102356AbcamRabbitGPhttp://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Guinea-pig-IgG-H-L-ab102356.html
H2AX560447BD PharmingenMouseHu 
Her-2MS-325-P0ThermoScientificMouseMshttp://www.thermoscientific.com/ecomm/servlet/productsdetail?productId=11954231&groupType=PRODUCT&searchType=0&storeId=11152&from=search
HIV1 p24ab19961AbcamGoatHuhttp://www.abcam.com/HIV1-p24-antibody-ab19961.html
HIV1 p24ab85536AbcamMouseHuhttp://www.abcam.com/HIV1-p24-antibody-24-2-ab85536.html
HLA-DRab92511AbcamRabbitHuhttp://www.abcam.com/hla-dr-antibody-epr3692-ab92511.html
HLA-DRM0746DakoMouseHuhttp://www.dako.com/us/ar38/p104640/prod_products.htm
ICOSab105227AbcamRabbitHuhttp://www.abcam.com/icos-antibody-sp98-ab105227.html
IL-17AF-317-NAR&D SystemsGoatHuhttp://www.rndsystems.com/Products/AF-317-NA
IL-1beta49-4960ProSciRabbitHuhttp://www.prosci-inc.com/shop/product.php?productid=33254&cat=0&page=1
InhibinPA0110LeicaMouseHuhttp://www.leicabiosystems.com/ihc-ish/novocastra-reagents/primary-antibodies/details/product/inhibin-alpha-1/
iNOSab15326AbcamRabbitHuhttp://www.abcam.com/inos-antibody-prediluted-ab15326.html
iNOSABN26MilliporeRabbitMs, Huhttp://www.emdmillipore.com/US/en/product/Anti-iNOSNOS-II-Antibody%2C-NT,MM_NF-ABN26
InsulinA0564DakoGuinea PigHuhttp://www.dako.com/us/ar38/p104840/prod_products.htm
InsulinI2018SigmaMouseRt, Hu, Rbhttp://www.sigmaaldrich.com/catalog/product/sigma/i2018?lang=en&region=US
Jak23230Cell SignalingRabbitMs, Hu, Rthttp://www.cellsignal.com/products/3230.html
Keratin 1905201BioLegendRabbitHu, Rt, Mk 
Keratin 14905301BiolegendRabbitHu, Ms, Rt, Dg, Mkhttp://www.biolegend.com/keratin-14-polyclonal-antibody-purified-antibody-10953.html
Ki-6712202Cell SignalingRabbitMshttp://www.cellsignal.com/products/primary-antibodies/ki-67-d3b5-rabbit-mab-mouse-preferred-ihc-formulated/12202
Ki-67M7240DakoMouseHuhttp://www.dako.com/us/download.pdf?objectid=104980004
Ly6BMCA771GABio-Rad AntibodiesRatMshttps://www.bio-rad-antibodies.com/mouse-ly-6b-2-alloantigen-antibody-7-4-mca771g.html?f=purified
Mac2CL8942APCedarlaneRatHu, Mshttp://www.cedarlanelabs.com/Products/Detail/CL8942AP
Mac2LS-B5661LS BioRabbitHu, Ms, Rthttp://www.lsbio.com/antibodies/anti-mac-2-galectin-3-antibody-internal-ls-b5661-ihc-plus/140267
Malondialdehydeab6463AbcamRabbitHuhttp://www.abcam.com/malondialdehyde-antibody-ab6463.html
MBPab7349AbcamRatHu, Ms, Rt, Rbhttp://www.abcam.com/myelin-basic-protein-antibody-12-oligodendrocyte-marker-ab7349.html
MCTMS-1216-POThermoScientificMouseHuhttp://www.thermoscientific.com/en/product/mast-cell-tryptase-ab-2-mouse-monoclonal-antibody.html
Mesothelin28127IBL AmericaRabbitMshttp://www.ibl-america.com/pdf/Other_Polyclonal_Antibodies/28127.pdf
MesothelinLS-C25601LS BioMouseHuhttp://www.lsbio.com/antibodies/anti-msln-antibody-mesothelin-antibody-if-immunofluorescence-ihc-wb-western-ihc-plus-ls-b2618/86124
MHC class II14-5321eBioscienceRatMshttp://us.ebioscience.com/mouse-mhc-class-ii-antibody-purified-m5-114152.htm
Mitofusin-1AP22961PU-NAcris AntibodiesGoatHu, Ms, Rt, Rbhttp://us.acris-antibodies.com/antibodies/mitofusin-1-ap22961pu-n.htm
MMP2818237-1-APProteinTechRabbitHu, Ms, Rthttp://www.ptglab.com/Products/MMP28-Antibody-18237-1-AP.htm
Mouse IsotypePA0996LeicaMouse http://www.leica-microsystems.com/fileadmin/img_uploads/novocastra_reagents/Novocastra_datasheets/pa0996.pdf
MPOa0398DakoRabbitHuhttp://www.dako.com/us/ar38/p105310/prod_products.htm
MPORB-373-A1ThermoScientificRabbitHu, Mshttp://www.thermoscientific.com/en/product/myeloperoxidase-mpo-rabbit-polyclonal-antibody.html
Muc1ab80952AbcamA. HamsterHu, Mshttp://www.abcam.com/muc1-antibody-mh1–same-as-ct2-ab80952.html
MyosinM8421SigmaMouseRb, Hu, Rt, Mshttp://www.sigmaaldrich.com/catalog/product/sigma/m8421?lang=en&region=US
N-Cadherinab18206AbcamRabbitMs, Rt, Huhttp://www.abcam.com/n-cadherin-antibody-ab18203.html
NF-KappaB1546-1EpitmoicsRabbitMs, Huhttp://www.epitomics.com/products/product_info/298/NF-&%23954%3bB-antibody-1546-1.html
Notch 13608Cell SignalingRabbitMs, HU, Rthttp://www.cellsignal.com/products/primary-antibodies/notch1-d1e11-xp-rabbit-mab/3608
      
Pancreatic Polypeptide (PP)NB100-1793NovusGoatHu, Mshttp://www.novusbio.com/Pancreatic-Polypeptide-PP-Antibody_NB100-1793.html
PDGFRb04-397MilliporeRabbitHu, Ms, Rthttp://www.millipore.com/catalogue/item/04-397
PDL1 (CD276)ab80276AbcamRatMshttp://www.abcam.com/pd-l1-antibody-mih6-low-endotoxin-azide-free-ab80276.html
PDL1 (CD276)ABC324MilliporeRabbitHuhttp://www.emdmillipore.com/US/en/product/Anti-PDL-1-Antibody,MM_NF-ABC324
PLA2R1HPA012657SigmaRabbitHuhttp://www.sigmaaldrich.com/catalog/product/sigma/hpa012657?lang=en&region=US
PPAR600-401-421Rockland Inc.RabbitMs, Rthttp://www.rockland-inc.com/store/Transcription-Factor-Antibodies-600-401-421-O4L_23035.aspx
proSP-CAB3786MilliporeRabbitRt, Hu, Mshttp://www.millipore.com/catalogue/item/ab3786
pSTATS2690SigmaRabbitHu, Ms, Rthttp://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/5/s2690dat.Par.0001.File.tmp/s2690dat.pdf
pSTAT39145Cell SignalingRabbitHu, Ms, Rthttp://www.cellsignal.com/products/9145.html
pSTAT59314Cell SignalingRabbitHu, Mshttp://www.cellsignal.com/products/9314.html
      
Rabbit IsotypeAB-105-CR&D SystemsRabbit http://www.rndsystems.com/product_results.aspx?k=AB-105-c
Rabbit SecondaryA16110Life TechnologiesGoatRabbithttps://www.lifetechnologies.com/order/catalog/product/A16110?ICID=search-a16110
Rat Isotype553986BD Pharmingen  http://www.bdbiosciences.com/ptProduct.jsp?prodId=3268
ROR1AF2000R&D SystemsGoatHu, Mshttp://www.rndsystems.com/Products/af2000/
S-10018-0046InvitrogenRabbitHu, Ms, Rthttp://www.lifetechnologies.com/order/catalog/product/180046?ICID=search-180046
Siglec-HNBP2-27062NovusRatMshttp://www.novusbio.com/Siglec-H-Antibody-23M15C8_NBP2-27062.html
Smooth Muscle ActinM0851DakoMouseHu, Mshttp://www.dako.com/us/ar38/p102030/prod_products.htm
Smooth Muscle Actinab32575AbcamRabbitHu, Ms, Rthttp://www.abcam.com/alpha-smooth-muscle-actin-antibody-e184-ab32575.html
Somatostatinsc-7819Santa Cruz BiotechnologyGoatHu, Rthttp://datasheets.scbt.com/sc-7819.pdf
SV40554149BD PharmingenMouseMshttp://www.bdbiosciences.com/ptProduct.jsp?prodId=12526&key=554149&param=search&mterms=true&from=dTable
Tyrosine HydroxylaseMAB318MilliporeMouseHu, Mshttp://www.millipore.com/catalogue/item/mab318
UPC1ab23841AbcamRabbitMs, Rthttp://www.abcam.com/ucp1-antibody-ab23841.html
Versicanab177480AbcamRabbitMs,Rt,Hu 
VimentinM0725DakoMouseHuhttp://www.dako.com/us/ar38/p106290/prod_products.htm
WGAW11261Life Technology  http://products.invitrogen.com/ivgn/product/W11261
WNT5AMA5-15511ThermoScientificMouseHuhttps://www.fishersci.com/ecomm/servlet/itemdetail?storeId=10652&langId=-1&catalogId=29104&productId=14233375&distype=0&highlightProductsItemsFlag=Y&fromSearch=1&searchType=PROD&hasPromo=0
WNVW1015VRLRabbitMs 
WT1APPB0991ThermoScientificRabbitHu, Ms, Rthttp://www.fishersci.com/ecomm/servlet/itemdetail?catnum=APPB0991&storeId=10652
WT1M356DakoMouseHuhttp://www.dako.com/us/download.pdf?objectid=106952002
      
YM1AF2446R&D SystemsGoatMshttp://www.rndsystems.com/pdf/AF2446.pdf