Histology and Imaging Core (HIC)

Optimized Antibodies

The HIC utilizes automated immunohistochemistry stainers which provide efficiency and consistency as well as standardization of protocols across studies of all sizes.  Please review the list of over 100 antibodies that have been optimized in our core.  

Please feel free to consult with HIC staff to discuss antibody selection, fixation techniques and control tissue for any IHC project.  Some antibodies are simply not effective for immunohistochemistry techniques and the HIC staff can help you determine the best marker for your study needs.   We are constantly optimizing new (to our core) antibodies so please contact Brian Johnson if an antibody you are interested in is not on our optimized list.

 

Antibody Catalog Number Company Raised in: Ractive to: Datasheet link
AF-488 A-11001 Life Technologies Goat Ms http://products.invitrogen.com/ivgn/product/A11001
AF-546 A-11010 Life Technologies Goat Rb http://products.invitrogen.com/ivgn/product/A11010?ICID=search-a11010
AF-594 A11062 Invitrogen Rabbit Ms http://products.invitrogen.com/ivgn/product/A11062?ICID=search-a11062
Annexin A2 60051-1-Ig ProteinTech Mouse Hu, Ms  
ANP ab91250 Abcam Rabbit Hu, Ms, Mk http://www.abcam.com/anp-antibody-n-terminal-ab91250.html
APOM LS-B5901 Lifespan Bioscience Goat Ms, Hu http://www.lsbio.com/Documents/PDF/Antibodies/141308
Arginase 1 sc-20150 Santa Cruz Biotechnology Rabbit Hu, Ms, Rt http://datasheets.scbt.com/sc-20150.pdf
Armenian Hamster 2° 127-035-160 Jackson ImmnunoResearch Goat A. Hamster https://www.jacksonimmuno.com/catalog/quickfind
B-Catenin ab16051 Abcam Rabbit Ms, Rt, Hu  
BrdU ab6326 Abcam Rat Ms http://www.abcam.com/BrdU-antibody-BU1-75-ICR1-ab6326.html
C-Fos PC38 Calbiochem Rabbit Hu,Ms,Rt http://www.ebiotrade.com/buyf/productsf/calbiochem/pc38.pdf
Calbindin 300 Swant Mouse Rb http://www.labome.com/product/SWant/300.html
Calbindin CB38 Swant Rabbit Mu, Ms, Rt, Mk http://1degreebio.org/reagents/product/504930/?qid=0
CAMP LS-C94098 Lifespan Bioscience Rabbit Ms http://www.lsbio.com/Documents/PDF/Antibodies/95319
CC3 CP229B BioCare Medical Rabbit Hu, Ms http://biocare.net/wp-content/uploads/229.pdf
CCR6 MAB195 R&D Systems Mouse Hu http://www.rndsystems.com/Products/MAB195
CD3 MCA1477 AbD Serotec Rat Hu, Ms http://www.abdserotec.com/catalog/datasheet-MCA1477.html
CD3 NCL-L-CD3-565 Leica Mouse Hu http://www.leicabiosystems.com/ihc-ish/novocastra-reagents/primary-antibodies/details/product/cd3-1/
CD4 550278 BD Pharmingen Rat Ms http://www.bdbiosciences.com/ptProduct.jsp?prodId=18261&key=550278&param=search&mterms=true&from=dTable
CD4 VP-C319 Vector Mouse Hu http://www.vectorlabs.com/data/protocols/VPC319.pdf
CD4 14-9766-80 eBioscience Rat Ms http://us.ebioscience.com/mouse-cd4-antibody-purified-4sm95.htm
CD8 ab4055 Abcam Rabbit Hu http://www.abcam.com/cd8-antibody-ab4055.html
CD8a 550281 BD Pharmingen Rat Ms http://www.bdbiosciences.com/ptProduct.jsp?prodId=18283&key=550281&param=search&mterms=true&from=dTable
CD8A 14-0808-80 eBioscience Rat Ms http://us.ebioscience.com/mouse-cd8a-antibody-purified-4sm15.htm
CD8 NCL-L-CD8-295 Leica Ms Hu http://www.leicabiosystems.com/ihc-ish-fish/immunohistochemistry-ihc-antibodies-novocastra-reagents/primary-antibodies/details/product/cd8/
CD11c bs-2508R Bioss Antibodies Rabbit Hu http://biossusa.com/store/bs-2508r.html
CD14 NCL-L-CD14-223 Leica Mouse Hu http://www.leicabiosystems.com/ihc-ish/novocastra-reagents/primary-antibodies/details/product/cd14/
CD20 NCL-L-CD20-L26 Leica Mouse Hu http://www.leicabiosystems.com/ihc-ish/novocastra-reagents/primary-antibodies/details/product/cd20/
CD31 ab56299 Abcam Rat Ms http://www.abcam.com/CD31-antibody-RM0032-1D12-ab56299.htm
CD31 PA0250 Leica Mouse Hu http://www.leica-microsystems.com/fileadmin/img_uploads/novocastra_reagents/Novocastra_datasheets/pa0250.pdf
CD31 DIA-310 Dianova Rat Ms http://www.dianova.com/en/products/product-highlights/murine-cd31-clone-sz31-formalin-fixed-paraffin-embedded-tissues/
CD45R 557390 BD Pharmingen Rat Ms http://www.bdbiosciences.com/ptProduct.jsp?prodId=881&key=557390&param=search&mterms=true
CD45R M0701 Dako Mouse Hu http://www.dako.com/us/ar38/p103020/prod_products.htm
CD66b 555723 BD Pharmingen Mouse Hu http://www.bdbiosciences.com/external_files/Doc_Recon_2.0/pm/tds/555723.pdf
CD68 MCA1957 AbD Serotec Rat Ms http://www.abdserotec.com/CodePages/Datasheet.aspx?ProductCode=MCA1957
CD68 PA0273 Leica Mouse Hu http://www.leica-microsystems.com/products/total-histology/novocastra-reagents/primary-antibodies/details/product/cd68/
CD138 (Syndecan-1) 553712 BD Pharmingen Rat Ms http://www.bdbiosciences.com/ptProduct.jsp?prodId=1716&key=553712&param=search&mterms=true&from=dTable
CD163 NCL-L-CD163 Leica Mouse Hu http://www.leicabiosystems.com/fileadmin/img_uploads/novocastra_reagents/Novocastra_datasheets/cd163-l-u.pdf
Claudin-3 PA5-16867 ThermoScientific Rabbit Hu http://www.pierce-antibodies.com/Claudin-3-antibody-Polyclonal–PA516867.html
Cleaved Notch 1 4147 Cell Signaling Rabbit Hu, Ms, Rt http://www.cellsignal.com/products/primary-antibodies/cleaved-notch1-val1744-d3b8-rabbit-mab/4147
CPA1 HPA008689 Sigma Rabbit Hu http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=HPA008689|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC
Cytokeratin WSS Z0622 Dako Rabbit Cow http://www.dako.com/us/ar38/p104910/prod_products.htm
Desmin M0760 Dako Mouse Hu http://www.dako.com/us/ar38/p104050/prod_products.htm
E-Cadherin 3195 Cell Signaling Rabbit Ms, Hu http://www.cellsignal.com/products/primary-antibodies/e-cadherin-24e10-rabbit-mab/3195?hit=productId&Ntt=3195
E. coli B0357 Dako Rabbit Hu http://www.dako.com/us/ar38/p235383/prod_products.htm
EGFr 28-005 Invitrogen Mouse Hu http://products.invitrogen.com/ivgn/product/280005?ICID=search-280005
ENT1 LSB3385/22621 Lifespan Bioscience Rabbit Hu http://www.lsbio.com/Products/Antibodies/AntibodyDetail.aspx?AntibodyID=106033
ESAT6 sc-57730 Santa Cruz Biotechnology Mouse ZF http://datasheets.scbt.com/sc-57730.pdf
Estrogen Receptor (ERa) A00010 ScyTek Mouse Hu  
F4/80 MF48000 Invitrogen Rat Ms http://www.invitrogen.com/site/us/en/home.html
FGF9 ab71395 Abcam Rabbit Ms, Hu http://www.abcam.com/fgf9-antibody-ab71395.html
FLAG 2368 Cell Signaling Rabbit Ms http://www.cellsignal.com/products/primary-antibodies/2368
Flu B65141G Meridian Life Science Goat Ms http://meridianlifescience.com/products/results_2.aspx?searchbox=b65141g&page=1&group=0
Foxp3 14-4777-82 eBioscience Mouse Hu  
Foxp3 ab20034 Abcam Mouse Hu http://www.abcam.com/foxp3-antibody-236ae7-ab20034.html
Foxp3 14-5773 eBioscience Rat Ms, Rt http://us.ebioscience.com/mouse-rat-foxp3-antibody-purified-fjk-16s.htm
Galectin3 PA0238 Leica Mouse Hu http://www.leica-microsystems.com/products/total-histology/novocastra-reagents/primary-antibodies/details/product/galectin-3-1/
GFAP Z0334 Dako Rabbit Ms www.dako.com/ar38/p235385/prod_products.htm
Glucagon G2654 Sigma Mouse Rt, Hu  
Glucagon G2654 Sigma Mouse Ms  
Goat Isotype 02-6202 Invitrogen Goat   http://products.invitrogen.com/ivgn/product/026202?CID=Search-02-6202
Goat Secondary 305-005-045 Jackson ImmnunoResearch Rabbit Goat http://www.jacksonimmuno.com/MERCHANT2/merchant.mv?Screen=PROD&Product_Code=305-005-045
Goat Secondary G4018 Sigma Rabbit Goat http://www.sigmaaldrich.com/catalog/product/sigma/g4018?lang=en&region=US
GR1 MAB1037 R&D Systems Rat Ms https://www.rndsystems.com/products/mouse-gr-1-ly-6g-antibody-rb6-8c5_mab1037
GranzymeB ab4059 Abcam Rabbit Hu, Ms, Rt http://www.abcam.com/granzyme-b-antibody-ab4059.html
Guinea Pig Secondary ab102356 Abcam Rabbit GP http://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Guinea-pig-IgG-H-L-ab102356.html
H2AX 560447 BD Pharmingen Mouse Hu  
Her-2 MS-325-P0 ThermoScientific Mouse Ms http://www.thermoscientific.com/ecomm/servlet/productsdetail?productId=11954231&groupType=PRODUCT&searchType=0&storeId=11152&from=search
HIV1 p24 ab19961 Abcam Goat Hu http://www.abcam.com/HIV1-p24-antibody-ab19961.html
HIV1 p24 ab85536 Abcam Mouse Hu http://www.abcam.com/HIV1-p24-antibody-24-2-ab85536.html
HLA-DR ab92511 Abcam Rabbit Hu http://www.abcam.com/hla-dr-antibody-epr3692-ab92511.html
HLA-DR M0746 Dako Mouse Hu http://www.dako.com/us/ar38/p104640/prod_products.htm
ICOS ab105227 Abcam Rabbit Hu http://www.abcam.com/icos-antibody-sp98-ab105227.html
IL-17 AF-317-NA R&D Systems Goat Hu http://www.rndsystems.com/Products/AF-317-NA
IL-1beta 49-4960 ProSci Rabbit Hu http://www.prosci-inc.com/shop/product.php?productid=33254&cat=0&page=1
Inhibin PA0110 Leica Mouse Hu http://www.leicabiosystems.com/ihc-ish/novocastra-reagents/primary-antibodies/details/product/inhibin-alpha-1/
iNOS ab15326 Abcam Rabbit Hu http://www.abcam.com/inos-antibody-prediluted-ab15326.html
iNOS ABN26 Millipore Rabbit Ms, Hu http://www.emdmillipore.com/US/en/product/Anti-iNOSNOS-II-Antibody%2C-NT,MM_NF-ABN26
Insulin A0564 Dako Guinea Pig Hu http://www.dako.com/us/ar38/p104840/prod_products.htm
Insulin I2018 Sigma Mouse Rt, Hu, Rb http://www.sigmaaldrich.com/catalog/product/sigma/i2018?lang=en&region=US
Jak2 3230 Cell Signaling Rabbit Ms, Hu, Rt http://www.cellsignal.com/products/3230.html
Keratin 1 905201 BioLegend Rabbit Hu, Rt, Mk  
Keratin 14 905301 Biolegend Rabbit Hu, Ms, Rt, Dg, Mk http://www.biolegend.com/keratin-14-polyclonal-antibody-purified-antibody-10953.html
Ki-67 12202 Cell Signaling Rabbit Ms http://www.cellsignal.com/products/primary-antibodies/ki-67-d3b5-rabbit-mab-mouse-preferred-ihc-formulated/12202
Ki-67 M7240 Dako Mouse Hu http://www.dako.com/us/download.pdf?objectid=104980004
Ly6B MCA771GA Bio-Rad Antibodies Rat Ms https://www.bio-rad-antibodies.com/mouse-ly-6b-2-alloantigen-antibody-7-4-mca771g.html?f=purified
Mac2 CL8942AP Cedarlane Rat Hu, Ms http://www.cedarlanelabs.com/Products/Detail/CL8942AP
Mac2 LS-B5661 LS Bio Rabbit Hu, Ms, Rt http://www.lsbio.com/antibodies/anti-mac-2-galectin-3-antibody-internal-ls-b5661-ihc-plus/140267
Malondialdehyde ab6463 Abcam Rabbit Hu http://www.abcam.com/malondialdehyde-antibody-ab6463.html
MBP ab7349 Abcam Rat Hu, Ms, Rt, Rb http://www.abcam.com/myelin-basic-protein-antibody-12-oligodendrocyte-marker-ab7349.html
MCT MS-1216-PO ThermoScientific Mouse Hu http://www.thermoscientific.com/en/product/mast-cell-tryptase-ab-2-mouse-monoclonal-antibody.html
Mesothelin 28127 IBL America Rabbit Ms http://www.ibl-america.com/pdf/Other_Polyclonal_Antibodies/28127.pdf
Mesothelin LS-C25601 LS Bio Mouse Hu http://www.lsbio.com/antibodies/anti-msln-antibody-mesothelin-antibody-if-immunofluorescence-ihc-wb-western-ihc-plus-ls-b2618/86124
MHC class II 14-5321 eBioscience Rat Ms http://us.ebioscience.com/mouse-mhc-class-ii-antibody-purified-m5-114152.htm
Mitofusin-1 AP22961PU-N Acris Antibodies Goat Hu, Ms, Rt, Rb http://us.acris-antibodies.com/antibodies/mitofusin-1-ap22961pu-n.htm
MMP28 18237-1-AP ProteinTech Rabbit Hu, Ms, Rt http://www.ptglab.com/Products/MMP28-Antibody-18237-1-AP.htm
Mouse Isotype PA0996 Leica Mouse   http://www.leica-microsystems.com/fileadmin/img_uploads/novocastra_reagents/Novocastra_datasheets/pa0996.pdf
MPO a0398 Dako Rabbit Hu http://www.dako.com/us/ar38/p105310/prod_products.htm
MPO RB-373-A1 ThermoScientific Rabbit Hu, Ms http://www.thermoscientific.com/en/product/myeloperoxidase-mpo-rabbit-polyclonal-antibody.html
Muc1 ab80952 Abcam A. Hamster Hu, Ms http://www.abcam.com/muc1-antibody-mh1–same-as-ct2-ab80952.html
Myosin M8421 Sigma Mouse Rb, Hu, Rt, Ms http://www.sigmaaldrich.com/catalog/product/sigma/m8421?lang=en&region=US
N-Cadherin ab18206 Abcam Rabbit Ms, Rt, Hu http://www.abcam.com/n-cadherin-antibody-ab18203.html
NF-KappaB 1546-1 Epitmoics Rabbit Ms, Hu http://www.epitomics.com/products/product_info/298/NF-&%23954%3bB-antibody-1546-1.html
Notch 1 3608 Cell Signaling Rabbit Ms, HU, Rt http://www.cellsignal.com/products/primary-antibodies/notch1-d1e11-xp-rabbit-mab/3608
           
Pancreatic Polypeptide (PP) NB100-1793 Novus Goat Hu, Ms http://www.novusbio.com/Pancreatic-Polypeptide-PP-Antibody_NB100-1793.html
PDGFRb 04-397 Millipore Rabbit Hu, Ms, Rt http://www.millipore.com/catalogue/item/04-397
PDL1 (CD276) ab80276 Abcam Rat Ms http://www.abcam.com/pd-l1-antibody-mih6-low-endotoxin-azide-free-ab80276.html
PDL1 (CD276) ABC324 Millipore Rabbit Hu http://www.emdmillipore.com/US/en/product/Anti-PDL-1-Antibody,MM_NF-ABC324
PLA2R1 HPA012657 Sigma Rabbit Hu http://www.sigmaaldrich.com/catalog/product/sigma/hpa012657?lang=en&region=US
PPAR 600-401-421 Rockland Inc. Rabbit Ms, Rt http://www.rockland-inc.com/store/Transcription-Factor-Antibodies-600-401-421-O4L_23035.aspx
proSP-C AB3786 Millipore Rabbit Rt, Hu, Ms http://www.millipore.com/catalogue/item/ab3786
pSTAT S2690 Sigma Rabbit Hu, Ms, Rt http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/5/s2690dat.Par.0001.File.tmp/s2690dat.pdf
pSTAT3 9145 Cell Signaling Rabbit Hu, Ms, Rt http://www.cellsignal.com/products/9145.html
pSTAT5 9314 Cell Signaling Rabbit Hu, Ms http://www.cellsignal.com/products/9314.html
           
Rabbit Isotype AB-105-C R&D Systems Rabbit   http://www.rndsystems.com/product_results.aspx?k=AB-105-c
Rabbit Secondary A16110 Life Technologies Goat Rabbit https://www.lifetechnologies.com/order/catalog/product/A16110?ICID=search-a16110
Rat Isotype 553986 BD Pharmingen     http://www.bdbiosciences.com/ptProduct.jsp?prodId=3268
ROR1 AF2000 R&D Systems Goat Hu, Ms http://www.rndsystems.com/Products/af2000/
S-100 18-0046 Invitrogen Rabbit Hu, Ms, Rt http://www.lifetechnologies.com/order/catalog/product/180046?ICID=search-180046
Siglec-H NBP2-27062 Novus Rat Ms http://www.novusbio.com/Siglec-H-Antibody-23M15C8_NBP2-27062.html
Smooth Muscle Actin M0851 Dako Mouse Hu, Ms http://www.dako.com/us/ar38/p102030/prod_products.htm
Smooth Muscle Actin ab32575 Abcam Rabbit Hu, Ms, Rt http://www.abcam.com/alpha-smooth-muscle-actin-antibody-e184-ab32575.html
Somatostatin sc-7819 Santa Cruz Biotechnology Goat Hu, Rt http://datasheets.scbt.com/sc-7819.pdf
SV40 554149 BD Pharmingen Mouse Ms http://www.bdbiosciences.com/ptProduct.jsp?prodId=12526&key=554149&param=search&mterms=true&from=dTable
Tyrosine Hydroxylase MAB318 Millipore Mouse Hu, Ms http://www.millipore.com/catalogue/item/mab318
UPC1 ab23841 Abcam Rabbit Ms, Rt http://www.abcam.com/ucp1-antibody-ab23841.html
Versican ab177480 Abcam Rabbit Ms,Rt,Hu  
Vimentin M0725 Dako Mouse Hu http://www.dako.com/us/ar38/p106290/prod_products.htm
WGA W11261 Life Technology     http://products.invitrogen.com/ivgn/product/W11261
WNT5A MA5-15511 ThermoScientific Mouse Hu https://www.fishersci.com/ecomm/servlet/itemdetail?storeId=10652&langId=-1&catalogId=29104&productId=14233375&distype=0&highlightProductsItemsFlag=Y&fromSearch=1&searchType=PROD&hasPromo=0
WNV W1015 VRL Rabbit Ms  
WT1 APPB0991 ThermoScientific Rabbit Hu, Ms, Rt http://www.fishersci.com/ecomm/servlet/itemdetail?catnum=APPB0991&storeId=10652
WT1 M356 Dako Mouse Hu http://www.dako.com/us/download.pdf?objectid=106952002
           
YM1 AF2446 R&D Systems Goat Ms http://www.rndsystems.com/pdf/AF2446.pdf